CRISPR Gene Editing: Using CRISPR-Cas9 with the Out of the Blue CRISPR Kit

Follow along with this step-by-step walkthrough of the lacZ gene editing laboratory activity in Bio-Rad’s Out of the Blue #CRISPR Kit. Click “Show More” for chapters and free resources. 👇 00:00 – Introduction 01:22 – lacZ in blue-white screening 02:57 – CRISPR-Cas9 gene editing 05:21 – Laboratory protocol overview and background 08:23 – Experimental setup 09:24 – Example Results 09:55 – Instructor Preparation 10:22 – Student workstation setup 11:17 – Student protocol 20:36 – Additional Information See the first video in this series CRISPR Explained: Gene Editing History, Technology, and Applications at For more videos about bacterial transformation visit the pGLO Bacterial Transformation playlist at The 2020 Nobel Prize in Chemistry was awarded for CRISPR. Learn more about the Out of the Blue CRISPR Kit and Genotyping Extension, and download free modeling and bioinformatics activities in the instructor and student guides, at Free CRISPR teaching resources at See more curated videos on our CRISPR Gene Editing playlist at *** Know of a YouTube video or channel helpful to life science students and teachers we could add to our Explorer Video Community? Email explorer@! *** *** Stay up-to-date with the latest videos added to the Explorer Video Community: subscribe to this YouTube channel at *** Bio-Rad Explorer’s YouTube channel is a hub of educational and instructional videos from Bio-Rad and the life science education community. Bio-Rad Explorer synergistically brings about change in hands-on science learning and provides educators with easy-to-use and reliable research-quality kits, equipment, reagents, and curricula, helping educators to stay on the cutting edge. ALL Bio-Rad products used for K-12 and undergraduate classes are eligible for the EDU discount. Technical Support and Customer Care: 1-800-4-BIORAD Website: Twitter: Pinterest: LinkedIn: Facebook: Instagram: @BioRadEducation Snapchat: @BioRadLabs #biorad #bioradeducation #bioradexplorer