Neuroblasts -- axon growth + glia

You can see some really nice axon growth here. You can almost see it feeling its way across the cover slip. Also of note here: we think that the larger crawling cells with a criss-crossing of microtubules (you can see them individually!) are glia. The quality on this one’s a little better because I figured out where the option was to compress it less. ======================= ======================= This video is from a series of experiments where I’m trying to characterize the neurons we see after extracting the cells from developing drosophila eggs. We’re not 100% sure what’s what, so take any conjecture with a grain of salt. Drosophila eggs were collected overnight (~15hr) and then harvested plated on a coverslip coated with ECM. They settled and did some initial growths for about 4 hours. Finally, this time-lapse was taken on a TIRF microscope over the next 8hr. The fluorescence is due to UAS-Jupiter (which binds microtubules) and is being