FIJI (ImageJ): Analysis of Scratch Assays, Wound Healing & Cell Migration [Wound Healing Size Tool]

Learn how to use FIJI (ImageJ) to analyze images of scratch wound healing or transwell migration assays. The plugin used (Wound Healing Size Tool) automatically recognizes wound healing size, corrects the average wound width by considering its inclination, and quantifies parameters such as: area, wound area fraction, average wound width, and width deviation of the wound images. It can be used for individual images (2D) or stacks (time-lapse). Timeline: 00:00 Introduction 00:10 Single image (2D) and Wound healing size tool 02:17 Brightfield/Phase contrast stack analysis with Wound healing size Stacks Tool 04:25 Fluorescence image stack & Wound healing size Stacks Tool 05:49 Wound healing size Manual tool The Wound Healing size tool can be installed from here: #wound-healing-size-tool. Choose the updated version for manual selections. Download tool in Macros folder (toolsets) of the . Unzip the Wound_heal