Isolation: CD133+ Liver Stem Cells For Clonal Expansion l Protocol Preview

Watch the Full Video at Isolation of CD133 Liver Stem Cells for Clonal Expansion - a 2 minute Preview of the Experimental Protocol C. Bart Rountree, Wei Ding, Hein Dang, Colleen VanKirk, Gay M. Crooks Pennsylvania State College of Medicine, Department of Pediatrics and Pharmacology; Pennsylvania State College of Medicine, Department of Pharmacology; University of California Los Angeles, School of Medicine, Department of Pediatrics; Here we describe the isolation of CD133 expressing liver stem cells and cancer stem cells from whole murine liver, a process that requires tissue digestion, cell enrichment, and flow cytometry isolation. We include methods for advanced single cell isolation and clonal expansion. Visit ?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000 videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500 institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE’s extensive library of 14,000 videos in their research,education and teaching. Subscribe to our channel: