Forensic genetics: Rape case investigation

Autoradiography is an imaging technique that uses radioactive sources contained within the exposed sample. In vitro autoradiography methods involve the isolation of cellular components such as DNA, RNA, proteins or lipids, followed by labeling with suitable radioisotopes. In in vivo autoradiography, radioisotopes are coupled with radioactive tracers and administered orally or via injection, and the distribution of radiation is evaluated in thin tissue or whole-body cryosections. In vivo autoradiography using laboratory animals is widely used in metabolic studies, disease monitoring and new drug development experiments. Common radioisotopes in autoradiography are sulfur-35, hydrogen-3, carbon-14, 125-iodine or phosphorus-32 (35S, 3H, 14C, 125I and 32P, respectively) which are used to determine the distribution of the radiolabeled molecules in tissues, cells or cellular organelles, but also in the study of protein modifications and DNA / RNA sequencing #Autoradiograph #DNA #ForensicScience #GelElectrophoresis