How to Model the Human Airway at the Air-Liquid interface: Introduction

In this series of videos, we will guide you through the protocols for expanding and differentiating human bronchial epithelial cells (HBECs) at the air-liquid interface (ALI). We will use the PneumaCult™ culture system to demonstrate ALI culture for this video. If you are using other culture media, some alterations to this protocol may be necessary. 00:00 Introduction 00:48 Overview of the ALI culture workflow using PneumaCult™ 01:02 Overview of phase 1 - Expansion culture in a flask 01:15 Overview of phase 2 - Submerged expansion in porous inserts 01:37 Overview of phase 3 - ALI culture differentiation in inserts Watch the other videos in this series: Video 1: How to Model the Human Airway at the ALI: Expansion of HBECs - Video 2: How to Model the Human Airway at the Air-Liquid Interface: Differentiation of HBECs - To learn more about PneumaCult™, visit us at #alicultures #airliquidinterface #pneumacult #airwayresearch For our full list of products visit our website Follow STEMCELL Technologies on Twitter at Like STEMCELL Technologies on Facebook at Subscribe to STEMCELL Technologies on YouTube at