Microscopy: Fluorescence Lifetime Imaging Microscopy (FLIM) (Philippe Bastiaens)

The fluorescence lifetime of a dye molecule is the amount of time that elapses between excitation of the dye and its emission of a photon Fluorescence lifetime imaging is the technique by which this lifetime is imaged and can be done using either widefield or confocal (time correlated single photon counting) methods. The fluorescence lifetime of a dye depends both on the dye and on the environment surrounding the dye. Because of this, FLIM can be a sensitive probe for environment as well as FRET. This lectu